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Smaller
vessels, as well as larger vessels which require 100+ samples/cycle,
present a new set of problems when it comes to retrieving a representative
sample. In comparison to more "standard" sampling scenarios,
there are either too few or no appropriate ports such as sanitary
connectors, ingold, or NA. As such, the technician must employ
some ingenuity to the situation. Most Bench-Top vessels (1 liter
- 15 liters) have a standard set of ports welded into the lid
of the vessel. One of these is a barbed spike connected to a
dip tube which extends into the vessel cavity. Generally there
is a length of tubing with one end sealed assembled onto the
barbed connector of the vessel.
The following is an overview of how a sample is
retrieved:
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Components:
- Vessel with blind tubing on barbed connector.
- Peristaltic pump.
- Pre-irradiated bag with whip of blind tubing.
- Tube welding device.
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Vessel with tubing length is autoclaved for sterility. |
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Tubing length is threaded into a peristaltic pump. |
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When sample is needed, tubing on vessel and whip on pre-irradiated
bag are spliced together using a machine such as the Terumo
TSCD tube welder. |
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When sterile fluid path is created, peristaltic pump extracts
sample through dip tube of vessel. |
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Finally, tube is sealed off and detached, giving the technician
a representative sample. |
ASI now offers a complete line of small vessel sampling bags
to compliment this procedure. As well, the patented “Pinch
and Cut” separation device negates the need for an expensive
tube sealing apparatus as described in the final step above.
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